Skip to main content
. 2016 Aug 16;7(36):58470–58482. doi: 10.18632/oncotarget.11298

Figure 4. The effect of EF2 overexpression on cell proliferation of NCI- H520 in vitro.

Figure 4

A. CCK-8 assay. Transfection of EF2 significantly promoted the growth of NCI- H520/pcDB-EF2 cells. B. Colony formation assay. The ratio of colony formation of NCI-H520/pcDB-EF2 cells was much higher than that of NCI-H520/pcDB cells (* p < 0.05). C. FACS analysis. Cell cycle distribution was determined by the DNA content. A demonstrated decreased percentage in the G2/M phase of the NCI-H520/pcDB-EF2 cells (* p < 0.05). D. Expression of Akt1, p-Akt, p-Cylin B1, p-Cdc2, EF2 and β-actin detected by western blot.