Figure 4. Effects of DSF/Cu pretreatment on NSCLC stem cell numbers in vitro and tumor seeding in vivo .
A. Schematic representation of treatment scheme. Cells were pretreated with drugs, then allowed to recover before experimental testing. B. Inhibitory effect of drug pretreatment on ALDH-positive cell populations before inoculation. C. Inhibitory effect of drug pretreatment on tumorsphere formation. Scale bar, 50 μm. (*P < 0.05, ***P < 0.001, χ2 test). D. Inhibitory effect of drug pretreatment on the expression of stem cell transcription factors. E. Inhibitory effect of drug treatment on transwell migration assays (**P < 0.01, ***P < 0.001, one-way ANOVA). F. Latency periods and tumor take percentage in xenograft mice receiving cells pretreated with the indicated drugs.