Figure 5.

Predictability observed in unbiased screens of higher eukaryotes. A CRISPR-Cas9 screen against four human cancer cell lines uncovered cell line-specific essential genes (Wang et al. 2014). (A) The specific hits for each cell line were used as the seed sets, and we found moderate predictability in three of the four cell lines, especially for KBM7. (B) Shown is the predictive functional net cluster induced by KBM7-specific hits, which are the boxed genes. Solid edges indicate connections in the human functional network, HumanNet. For clarity, only edges above a LLS weight cutoff of 3.0 and genes with more than one interaction to the hits are shown.