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. 2016 Dec 28;7(2):741–753. doi: 10.1534/g3.116.037192

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Copyright © 2017 Cahill et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Oligohistidine pulldown of spanin mutants. (A) Cartoon of Rz structure showing the relative location of CC1 mutants used in the oligohistidine pulldown. The TMD, and proximal and distal helices are represented with gray and striped boxes, respectively. Red lines below CC1 represent the relative position of mutant residues used in the pulldown assay. (B) Coimmunoprecipitation of Rz CC1 mutants with Rz1-His by oligohistidine pulldown. Pulldown products were analyzed by Western blot with anti-Rz and anti-Rz1 antibodies. (C) Cartoon of Rz structure showing the relative location of CC2 mutants used in the oligohistidine pulldown. The TMD, and proximal and distal helices are represented with gray, and striped boxes, respectively. Red lines below CC2 represent the relative position of mutant residues used in the pulldown assay. (D) Coimmunoprecipitation of Rz CC2 mutants with Rz1-His by oligohistidine pulldown. Pulldown products were analyzed by Western blot with anti-Rz and anti-Rz1 antibodies. (E) Cartoon of Rz1-His showing relative location of mutants used in the oligohistidine pulldown. Red lines below Rz1-His represent the relative position of mutant residues of Rz1-His used in the pulldown assay. This position of the His tag is represented with a green box. (F) Coimmunoprecipitation of Rz1-His mutants with Rz. Pulldown products were analyzed by Western blot with anti-Rz and anti-Rz1 antibodies.