| Bacteriophages |
|
|
| λ900 |
λΔ(stf tfa)::cat cI857 bor::kan; carries CamR and KanR
|
Laboratory stock |
| λ900RzQ100am Rz1+
|
Rz gene carries amber codon at position 100 with silent change in embedded Rz1
|
Laboratory stock |
| λ900Rz+Rz1W38am
|
Rz1 gene carries amber codon at position 38 with silent change in overlapping Rz
|
Laboratory stock |
| λ900RzQ100am Rz1W38am
|
|
Laboratory stock |
| Strains |
|
|
| MC4100tonA::Tn10
|
Escherichia coli K-12 F araD139 Δ(argF-lac)U169 rpsL15 relA1 flbB3501 deo pstF25 rbsR tonA
|
Laboratory stock |
| MC4100 λ900 |
MC4100 tonA::Tn10 lysogenized with λ900 |
Laboratory stock |
| MC4100 λ900RzQ100am Rz1+
|
MC4100 tonA::Tn10 lysogenized with λ900 RzQ100am Rz1+
|
Laboratory stock |
| MC4100 λ900Rz+Rz1W38am
|
MC4100 tonA::Tn10 lysogenized with λ900 Rz+Rz1W38am
|
Laboratory stock |
| MC4100 λ900RzQ100am Rz1W38am
|
MC4100 tonA::Tn10 lysogenized with λ900 RzQ100am Rz1W38am
|
Laboratory stock |
| RY17341 |
MDS12ΔtonA; MG1655 with 12 deletions, totaling 376,180 nt, including cryptic prophages |
Laboratory stock |
| RY17341 λ |
RY17341 lysogenized with temperature sensitive λcI857 |
This study |
| RY17299 lacIq1
|
Derived from MG1655 ΔtonA
|
Park et al. (2006) |
| Plasmids |
|
|
| pRE |
Plasmid with the λ later promoter pR’ that is transcriptionally activated by λQ |
Laboratory stock |
| pRz |
pRE carrying Rz alone with Rz1 inactivated by a nonsense mutation |
Laboratory stock |
| pRz1 |
pRE carrying Rz1 |
Laboratory stock |
| pRz mutX |
pRE carrying denoted mutation of Rz |
This study |
| pRz1 mutX |
pRE carrying denoted mutation of Rz1 |
This study |
| pSynRz |
pRE carrying Rz alone. The former region of Rz1 overlap within Rz was codon-optimized to be genetically disparate from WT Rz1 to avoid recombination |
This study |
| pSynRz1 |
pRE carrying Rz1 alone. SynRz1 was codon-optimized to be genetically disparate from WT Rz to avoid recombination |
This study |
| pSynRz mutX |
pRE carrying denoted mutation within SynRz |
This study |
| pSynRz1 mutX |
pRE carrying denoted mutation within SynRz1 |
This study |
| pSynRz Linker 101–115 |
Residues 101–115 of Rz replaced with Gly-Ser repeats |
|
| pLinkerRz Q100S |
Residues 100–115 of Rz replaced with eight Ser-Gly repeats |
This study |
| pRz1 25–30 GS-Linker |
Residues 25–30 of Rz1 replaced with 3 Gly-Ser repeats |
This study |
| pRz1his
|
Rz1 with His-tag at the C- terminal end |
Berry et al. (2008) |
| pQ |
pSC101 origin with low-copy mutation; Q cloned under Plac/ara-1 promoter |
Gründling et al. (2001) |
