Table 3. List of Rz and Rz1 mutants grouped by their substructural regions.

Rz Mutants					Rz1 Mutants
Codon	Change	Isolates	Lysis	Region	Codon	Change	Isolates	Lysis	Region
1	Met → Lys	1	−	TMD	1	Met → Lys	1	−	Signal sequence
4–24	Artificial TMD	0	+		12	Met » Arg	3	−
14	Cys → Arg	2	−		19	Gly » Cys	3	−
19	Leu → Pro	2	−		20	Cys » Ser	5	−
20	Ser → Pro	0	+		20	Cys → Arg	1	−
36	Gln → Pro	0	+	CC1	20	Cys » Phe	2	−
50	Ala → Pro	0	+		20	Cys → Tyr	3	−	L
57	Arg → Cys	2	−		25–30	(Gly-Ser)3	0	+
59	Arg → Cys	1	−		32	Pro » Gln	1	−
61	Val → Ala	1	−		33	Pro → Leu	1	−
62	Ala → Pro	0	−		35	Pro » His	3	−
64	Leu » His	2	−		36	Pro » Gln	3	−
64	Leu → Pro	3	−		36	Pro → Leu	2	−
64	Leu » Arg	1	−		39	Ile → Val	1	−	Proline-rich-region
65	Asp → Gly	2	−		42	Pro → Ser	1	−
65	Asp → Asn	1	−		44	Pro → Ser	1	−
65	Asp » Val	1	−		45	Asp → Lys	0	+
66	Ala » Pro	1	−		46	Trp » Cys	1	−
67	Lys » Asn	1	−		46	Trp → Arg	2	−	CTD
70	Lys → Glu	1	−		50	Leu » Arg	1	−
72	Leu » Phe	1	−		50	Leu → Pro	7	−
72	Leu → Ser	1	−		54	Ile » Asn	1	−
73	Ala » Pro	1	−		54	Ile → X(Ochre)	0	−
73	Ala → Val	1	−		55	Ser → X(Amber)	0	+
77	Ala » Pro	1	−		56	Pro → X(Opal)	0	+
82	Leu → Pro	1	−		57	Ser → X(Ochre)	0	+
83	Arg » Pro	1	−		58	Glu → Lys	1	−
86	Val » Gly	1	−		58	Glu → X (Ochre)	0	+
88	Ala » Pro	2	−		59	Arg → Glu	0	+
91	Arg » Pro	2	−	L1	59	Arg → X (Ochre)	0	+
93	Leu → Ser	3	−		60	Gly → X (Ochre)	0	+
100–115	(Gly-Ser)8	0	+
107	Thr → Pro	0	+
125	Arg → Pro	0	+
127	Tyr » Asn	1	−	CC2
134	Leu → Pro	3	−
141	Leu → Pro	1	−
143	Gly » Arg	1	−
147	Tyr → His	1	−
147	Tyr → Cys	2	−
150	Glu → Arg	0	−
150	Glu → Gly	0	−
151	Gln → Arg	2	−
151	Gln » Lys	1	−
151	Gln → Pro	0	+	CTD
151	Gln → X (Ochre)	0	−
152	Cys → X (Ochre)	0	−
153	Arg → X (Ochre)	0	+

The types of residue change, number of isolates obtained by random mutagenesis (positive integers), lysis function, and the relevant structural region of the mutant position are indicated in the table. A type of substitution between residue is indicated by an arrow symbol (→) for transition and by a double greater sign (») for transversion. Mutants indicated in bold were tested for their dominance in the presence of the corresponding wild-type allele. The ability of each allele either to support or block lysis by complementation is indicated by “+” or “−” symbol, respectively. Mutants created by site-directed mutagenesis are indicated by a “0” isolate number. Nonsense mutations are indicated by “X” and their type in parenthesis.