Figure 1.

Noncanonical NF-κB signaling pathway. (A) Schematic depiction of the function and key proteins of noncanonical NF-κB signaling. Proteins used in this study are marked by a red asterisk. (B) HEK293 Flp-In stable cell line system expressing SH-tagged NIK^wt, NIK^K429A/K430A, and NIK^P565R upon doxycycline induction. IB showing expression of NIK upon doxycycline treatment and NF-κB2/p100 processing due to expression of NIK^wt. This is a reduced depiction of the IB shown in Supplementary Figure S2B. Gray dotted lines indicate IB lanes that are not adjacent to one another.