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. 2016 Jul 15;15(9):2900–2909. doi: 10.1021/acs.jproteome.5b01004

Figure 3.

Figure 3

Validation of interacting proteins identified by LC–MS. (A) Co-IP using SH-GFP doxycycline-inducible control cell line, SH-TRAF2 and SH-NF-κB2/p100-inducible HEK293 Flp-In lines concomitantly expressing myc-FBXW7. IP with anti-HA agarose beads confirmed the association of SH-TRAF2 and SH-NF-κB2/p100 with myc-tagged FBXW7. (B) Co-IP with SH-GFP doxycycline-inducible control cell line, SH-IKKα and SH-NIK doxycycline-inducible HEK293 Flp-In lines. IP with anti-HA agarose beads confirmed the association of SH-IKKα and SH-NIK with endogenously expressed FKBP8. Gray dotted lines indicate lanes that are not adjacent to one another.