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nNOS-bound CaM dynamics and reaction chemistry are kinetically coupled. The graph shows the transient reaction chemistry of nNOS (relative absorbance, red) and dynamics of nNOS-bound CaM (relative FRET efficiency, grey) recorded when mixing excess NADPH with nNOS (bound to CaM), under pseudo first order conditions. The schematic presented here shows the temporal resolved dynamics of nNOS-bound CaM when nNOS is reduced with NADPH. This figure is adapted in part from Ref. [43].
