Fig. 5.

CPR domain dynamics and reaction chemistry are kinetically coupled. The graph shows the transient reaction chemistry (relative absorbance, red) and dynamics (relative FRET efficiency, blue) of CPR when mixing NADPH with the enzyme under pseudo first order conditions. The schematic shows the structures of the ‘open’ (PDB ID 3ES9) and ‘closed’ (PDB ID 1AMO) CPR structures with the FAD and FMN binding domains in dark blue and light blue, respectively. This figure is adapted in part from Ref. [34].