Fig 8. Evaluation of vaccine-mediated protection against L. infantum infection in BALB/c mice 1 month post intravenous challenge.

Determination of parasite load in (A) the liver and (B) the spleen by limiting dilution assay in vaccinated and non-vaccinated mice 1 month post challenge with L. infantum. Results are presented as means±SD of five individual mice per group, representative of two independent experiments with similar results. *p<0.05, **p<0.01, ***p<0.001 were assessed by one-way ANOVA and Tukey’s multiple comparison tests.