Figure 4.

Two-photon fluorescence images illustrating the detailed structure of mouse ONH astrocytes remaining stable for 3 hours in control GLT1/GFP explants (A, C = lower magnification; B, D = higher magnification) at 10 mm Hg. When IOP was increased from 10 mm Hg (E, F) to 30 mm Hg (G, H) for 2 hours, visible changes were detected in astrocyte somal shape (F, H: astrocyte soma elongates) and process orientation (E, G: astrocyte process straightens; arrows; magnification factor bar indicates 100 μm in A and C and 20 μm in B, D–H).