Skip to main content
. Author manuscript; available in PMC: 2017 Feb 7.
Published in final edited form as: Gastroenterology. 2012 Apr 10;143(1):223–233.e9. doi: 10.1053/j.gastro.2012.04.006

Figure 1.

Figure 1

Positions 447, 458, and 478 confer enhanced viral entry of a high-infectivity variant reinfecting the liver graft. (A) Genomic organization and mutations of envelope glycoproteins of escape variant VL and nonselected variants VC and VA. HVR1 and HVR2 are depicted in green; E2 domains are depicted in red (DI), yellow (DII), and blue (DIII); and CD81 binding domains are depicted in dark blue.29,33,39 Positions 447, 458, and 478 are highlighted in black vertical lines. Differences between VL, VC, and VA in region E1E2384–483 are displayed. (B and C) Viral entry in Huh7.5.1 cells of the escape variant VL, the nonselected variants VC and VA, as well as chimeric variants containing defined mutations of VC and VA in VL or vice versa (Supplementary Figure 1). HCVpp infection was analyzed by luciferase reporter gene expression. Results are expressed as the percentage of viral entry compared with VL. Means ± standard deviation from at least 4 independent experiments performed in triplicate are shown. Significant differences in HCVpp entry between variants are indicated (*P ≤ .05; **P < .001). aa, amino acid; BD, binding domain.