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. Author manuscript; available in PMC: 2017 Feb 7.
Published in final edited form as: Gastroenterology. 2012 Apr 10;143(1):223–233.e9. doi: 10.1053/j.gastro.2012.04.006

Figure 3.

Figure 3

Different CD81 use of viral variants in Huh7.5 cells with silenced CD81 expression. (A) Entry factor expression in Huh7.5 cells with silenced CD81 (grey bars) or CD13 (black bars) expression. CD81 expression was determined by flow cytometry and is indicated as fold expression compared with control shCD13-Huh7.5 cells. (B and C) Entry of patient-derived HCVpp VL, VC, and VA. Huh7.5 cells with silenced CD81 or CD13 expression were incubated with parental or chimeric HCVpp and viral entry was determined as described in Figure 1. Viral entry is expressed as the fold-change of viral entry compared with shCD13-Huh7.5 control cells. Means ± standard deviation from 3 independent experiments performed in triplicate are shown. Significant differences in HCVpp entry between wild-type and chimeric variants are indicated (**P < .001). (D) Entry kinetics of patient-derived variants. Kinetics of HCVpp entry was performed using anti-CD81 or isotype control antibody (5 μg/mL). HCV entry was determined as described in Figure 1. One representative experiment of 4 is shown.