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. Author manuscript; available in PMC: 2017 Feb 7.
Published in final edited form as: Gastroenterology. 2012 Apr 10;143(1):223–233.e9. doi: 10.1053/j.gastro.2012.04.006

Figure 4.

Figure 4

Positions 447, 458, and 478 mediate viral escape from neutralization by autologous transplant serum. Neutralization of the escape variant VL, variants VC and VA, and the chimeric strains. HCVpp were incubated with autologous anti-HCV–positive or control serum in serial dilutions for 1 hour at 37°C before incubation with Huh7.5.1 cells. Neutralization titers obtained by end point dilution are indicated. Dotted line indicates the threshold for a positive neutralization titer (1/40). Means ± standard deviation from at least 4 experiments performed in triplicate are shown. (A) Neutralization of variants VL, VL containing individual or combined mutations of VC, and VC with double substitutions of VL by autologous anti–HCV-positive pretransplant serum. (B) Neutralization of variants VL, VL containing individual mutations of VA, and VA with single substitution of VL by autologous anti-HCV–positive pretransplant serum. Significant differences in neutralization between variants are indicated (*P ≤ .05; **P < .001).