Extended Data Figure 5.

Cardiac defects in Strn3, Atg3, and Slc39a8 mutant embryos a,b severe fetal edema and sporadic hemorrhaging in E15.5 homozygous mutant embryos versus controls (n = 7 mutants analyzed) c,d Subtle, but consistent cardiac septal defects (arrowhead) observed transverse micro-CT volume sections in Strn3^−/− embryos (d) versus control (c) (n = 5 mutants analyzed). Atg3^+/− (e) and Atg3^−/− (f) E14.5 embryos imaged by micro-CT after contrast staining showing evidence of novel heart morphological defects including ventricular septal defects (white arrows in f). Atg3^−/− mice also show abnormal atrio-ventricular valves. (n = 4 mutants analyzed). Transverse (g,h) and coronal (i,j) sections through micro-CT volumes of mutant and control Slc39a8 E14.5 embryos reveals novel heart morphological defects including ventricular septal defects (white arrows in h). Slc39a8^−/− mice also show the absence of sternum, a small chest cavity and a small liver (j) (n = 4 mutants analyzed).