Figure 1. related to Figure S1. A modest increase in Plk4 promotes centrosome amplification and aneuploidy in vitro.

(A) System used for doxycycline-inducible expression of Plk4.

(B) Quantification of the level of centrosomal Plk4 in Plk4^Dox MEFs. N = 3, >150 centrosomes per experiment.

(C) Quantification of the level of centrosome amplification in Plk4^Dox MEFs. N = 3, >150 cells per experiment.

(D) Immunofluorescent images of centrosomes in Plk4^Dox MEFs.

(E) Quantification of anaphase phenotypes in Plk4^Dox MEFs. N = 3, >150 cells per experiment.

(F) Quantification of anaphase lagging chromosomes in Plk4^Dox MEFs. N = 3, >150 cells per experiment.

(G) Quantification of the fraction of cells having <2 or >2 copies of chromosome 15 or 16. N = 3, >150 cells per experiment.

(H) Immunofluorescent images of FISH performed on Plk4^Dox MEFs using probes against chromosome 15 and 16. Arrowheads mark each copy of Chr15 or Chr16.

(I) Graph showing the fold increase in cell number for Plk4^Dox MEFs. N = 3, performed in triplicate.

(J) Graph showing the fold increase in cell number for Plk4^Dox MEFs expressing SpCas9 and an sgRNA against p53. N = 3, performed in triplicate.

All data represent the means ±SEM. ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001; two-tailed Student’s t-test. Scale bars represent 10 μm.