Figure 2. related to Figure S2 and S3. Increased Plk4 levels promote chronic centrosome amplification in multiple tissues.

(A) Fold increase in Plk4 mRNA in tissues from Plk4^Dox mice treated with doxycycline for 1 month. N = 3, performed in triplicate.

(B and C) Quantification of the level of centrosome amplification in tissues from Plk4^Dox mice treated with doxycycline for 1 or 8 month. N ≥ 4.

(D) Quantification of centrosome number in tissues from Plk4^Dox mice treated with doxycycline for 8 months. N = 4.

(E) Representative images of centrosomes in tissues from doxycycline treated Plk4^Dox or control animals.

(F–H) Quantification of the fraction of cleaved caspase 3, TUNEL, or Ki67 positive cells in tissues from Plk4^Dox mice treated with doxycycline for 1 month. Data are means ±SEM (N = ≥4).

All data represent the means ±SEM. ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001; two-tailed Student’s t-test. Scale bars represent 10 μm.