Table 4.

Functional amino acid contents of soybean in response to the presence and absence of biochar and G. geotrichum when applied individually and in combination

Treatment	Asp (µg/g)	Thr (µg/g)	Met (µg/g)	ILE (µg/g)	Ser (µg/g)	Glu (µg/g)	Leu (µg/g)	Tyr (µg/g)	Gly (µg/g)	Phe (µg/g)	Lys (µg/g)	Cys (µg/g)	Val (µg/g)	His (µg/g)	Arg (µg/g)	Pro (µg/g)	Total amino acids (µg/g)
Control	20.73±2.13d	7.09±0.37c	1.52±0.56c	11.42±0.71b	4.64±0.84d	13.96±1.96d	24.16±2.10b	5.37±0.22a	5.70±0.38d	11.39±1.94ab	8.62±0.62ab	2.78±0.63d	10.34±1.51b	3.88±0.41b	10.59±0.92c	7.86±2.81b	150.05c
GG	45.70±5.95a	11.51±1.15a	1.79±0.49b	11.79±1.32ab	9.03±0.75a	20.55±1.45b	26.42±2.64b	5.79±0.14a	8.28±0.15b	8.36±1.64b	13.00±1.02a	2.86±0.73c	14.02±3.85a	5.30±0.24a	15.14±1.94b	10.66±2.26a	210.05a
BC	31.99±3.78c	9.51±0.76b	1.41±0.07d	8.28±0.96c	5.99±0.25c	18.04±1.15c	30.49±2.90a	0.00±0.00b	6.39±0.95c	11.03±1.30ab	7.08±0.29b	3.57±0.55a	14.51±2.75a	5.23±0.34a	15.42±2.26b	10.42±1.05a	179.36b
BC+GG	36.12±4.71b	9.62±1.45b	2.12±0.86a	12.45±1.24a	6.26±0.31b	30.66±1.76a	22.95±0.00c	0.00±0.00b	11.52±1.08a	12.22±1.17a	9.80±0.68ab	3.66±0.35a	14.98±2.94a	6.18±0.47a	17.07±1.16a	11.32±2.65a	206.57a

BC: biochar; GG: G. geotrichum; Asp: aspartic acid; Thr: threonine; Met: methionine; ILE: isoleucine; Ser: serine; Glu: glutamic acid; Leu: leucine; Tyr: tyrosine; Gly: glycine; Phe: phenylalanine; Lys: lysine; Cys: cysteine; Val: valine; His: histidine; Arg: arginine; Pro: proline. The amino acids were analyzed from freeze-dried soybean plants in the presence and absence of biochar and G. geotrichum applied individually and in combination, to identify the beneficial effects of their interactions. Each value is expressed as mean±SD of six replicates from three independent experiments. The data were analyzed by ANOVA using SAS. Values in a column followed by different letters show the significant differences (P<0.05) among treatments based on DMRT