Table 5.
Isoflavone contents of soybean in response to the presence and absence of biochar and G. geotrichum applied individually and in combination
Treatment | Isoflavone aglycones (μg/g) |
Malonyl isoflavones (μg/g) |
Acetyl isoflavones (μg/g) |
Isoflavone glucosides (μg/g) |
Total (μg/g) | |||||||
Daidzein | Glycitein | Genistein | Daidzin | Glycitin | Genistin | Daidzin | Genistin | Daidzin | Glycitin | Genistin | ||
Control | 95.40±0.03c | 144.07±0.04b | 1.11±0.67a | 4.53±0.08c | 20.07±0.88b | 31.80±0.42c | ND | ND | ND | 0.00±0.00c | 0.60±0.09a | 297.58±55.74b |
GG | 95.85±0.17c | 148.81±0.22b | ND | 4.45±0.00c | 20.31±1.03b | 34.15±0.24c | ND | ND | ND | 2.61±0.06b | ND | 306.18±58.84b |
BC | 111.15±0.08a | 183.30±0.26a | ND | 11.99±0.02b | 24.64±0.06a | 42.81±0.10b | ND | ND | ND | 3.86±0.24a | ND | 377.75±70.38a |
BC+GG | 105.18±0.07b | 148.00±0.31b | ND | 29.37±0.20a | 21.51±0.21b | 77.00±0.35a | ND | ND | ND | 4.16±0.19a | ND | 385.22±55.69a |
BC: biochar; GG: G. geotrichum; ND: not detected or not determined in the samples. The isoflavones were analyzed from freeze-dried soybean plants in the presence and absence of biochar and G. geotrichum applied individually and in combination, to identify the beneficial effect of their interaction. Each value is expressed as mean±SD of six replicates from three independent experiments. The data were analyzed by ANOVA using SAS. Values in a column followed by different letters show the significant differences (P<0.05) among treatments based on DMRT