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. Author manuscript; available in PMC: 2018 Feb 15.
Published in final edited form as: J Immunol. 2017 Jan 9;198(4):1748–1758. doi: 10.4049/jimmunol.1601750

Figure 2. Development of a quality control score to evaluate and correct for technical variation between experiment runs.

Figure 2

Cell frequencies were determined with the 10-color flow cytometry panel and the centralized manual gating method on 24 repeat runs or 10 provoked fail runs of cryopreserved PBMC derived from a single blood donation of a control donor. Quality control (QC) score was calculated based on the deviation from average for each of the normally distributed lymphocyte populations and 10% defined as the pass/fail threshold. (A) Quality control score obtained with normal repeat runs (black) or provoked fail runs (blue). (B) Detailed protocol and associated QC score for each provoked fail run. Effect of fixation with paraformaldehyde (PFA) on the frequency of (C) monocytes and (D) CD8 T cells, from three independent repeat runs of the control donation.