Table I.
Lymphocyte populations derived from the 10-color flow cytometry panel.
| Category | Gate ID | Population Name | Gating strategy | Resolution sensitivity |
|---|---|---|---|---|
| Major cell subsets | 5 | Monocytes | CD14+ | Clearly defined |
| 7 | B cells | CD14−/CD19+CD3− | Clearly defined | |
| 8 | NK cells | CD14−/CD56+CD3− | Clearly defined | |
| 11 | T cells | CD14−/CD56−CD3+ | Clearly defined | |
| 12 | CD4 T cells | CD14−/CD56−CD3+/CD4+CD8− | Clearly defined | |
| 13 | CD8 T cells | CD14−/CD56−CD3+/CD4−CD8+ | Clearly defined | |
| CD4 T cell subsets | 14 | Tregs | CD14−/CD56−CD3+/CD4+CD8−/CD25+ | Poorly resolved |
| 15 | Naïve CD4 T cells | CD14−/CD56−CD3+/CD4+CD8−/CD45RA+CCR7+ | Clearly defined | |
| 16 | Tcm CD4 T cells | CD14−/CD56−CD3+/CD4+CD8−/CD45RA−CCR7+ | Poorly resolved | |
| 17 | Tem CD4 T cells | CD14−/CD56−CD3+/CD4+CD8−/CD45RA−CCR7− | Poorly resolved | |
| 18 | Temra CD4 T cells | CD14−/CD56−CD3+/CD4+CD8−/CD45RA+CCR7− | Poorly resolved | |
| CD8 T cell subsets | 19 | Naïve CD8 T cells | CD14−/CD56−CD3+/CD4−CD8+/CD45RA+CCR7+ | Clearly defined |
| 20 | Tcm CD8 T cells | CD14−/CD56−CD3+/CD4−CD8+/CD45RA−CCR7+ | Poorly resolved | |
| 21 | Tem CD8 T cells | CD14−/CD56−CD3+/CD4−CD8+/CD45RA−CCR7− | Poorly resolved | |
| 22 | Temra CD8 T cells | CD14−/CD56−CD3+/CD4−CD8+/CD45RA+CCR7− | Poorly resolved | |
| CD56 T cell subsets | 9 | CD3+ CD56 T cells | CD14−/CD56+CD3+ | Poorly resolved |
| 10 | CD3hi CD56 T cells | CD14−/CD56+CD3hi | Poorly resolved |