Figure 3.

Bile duct ligation induces increased expression of autophagic proteins in PiZZ mouse liver. (A, B) Western blots of total liver tissue lysates showing increased Atg5, Atg12, and LC3A/B after BDL compared to sham livers. Ponceau is shown to confirm equal loading, since cytosketetal changes can affect both actin and tubulin in PiZZ mouse liver. (C) Confocal immunofluorescence microscopy showing the majority of LC3B (green) does not colocalize with GC cells labeled with anti-human A1AT (red) antibody (magnification: 100×). (D) Quantitative immunofluorescence data for LC3 and anti-human A1AT. Graph depicts a small subset of percentage of LC3⁺ cells colocalizing with ATZ GC cells (normalized to total number of cells).