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. Author manuscript; available in PMC: 2018 Feb 15.
Published in final edited form as: J Immunol. 2017 Jan 13;198(4):1460–1473. doi: 10.4049/jimmunol.1601415

Table 2.

Summary of sequencing and sequence processing results.

Subject ID Cells Raw reads Final analyzed naïve sequencesa Final analyzed memory sequencesa
Naïve Memory Naïve Memory IgM IgK IgL IgM IgG IgA IgK IgL
AR02 143K 66K 939,295 918,374 3,739 3,382 2,481 2,794 1,539 2,990 10,139 7,501
AR03 154K 12K 921,768 770,573 3,194 1,713 1,099 368b 253b 366b 1,032b 1,124b
AR04 324K 39K 995,808 541,605 47,577 19,034 11,417 4,216 1,689 2,290 9,003 6,340
AR05 140K 77K 741,561 743,956 30,648 16,579 11,008 13,502 1,485 4,234 19,022 11,681
MK02 57K 50K 894,106 787,754 427b 327b 162b 6,479 927 888 6,753 3,413
MK03 151K 126K 1,076,632 810,841 1,685 3,806 1,307 969 293 697 7,341 3,353
MK04 59K 7K 958,717 398,324 8,123 5,180 3,101 543b 105b 257b 1,056b 687b
MK05 410K 192K 1,162,652 1,048,316 8,949 11,100 6,148 4,296 1,146 1,954 23,701 12,297
MK08 50K 25K 916,527 898,976 3,236 2,115 1,579 864 436 1,609 3,390 3,110
HD07 45K 16K 814,996 725,940 830b 838b 604b 3,004 586 1,891 4,937 3,737
HD09 171K 38K 1,332,467 535,370 14,079 14,124 7,304 2,167 501 954 9,021 4,616
HD10 80K 57K 1,080,339 774,156 4,513 3,878 2,612 3,101 548 446 5,926 4,723
HD13 89K 21K 933,799 401,302 6,262 7,732 3,224 2,552 1,223 1,265 7,467 4,585
a

Refers to the count of unique, error-corrected sequences which passed all quality control and filtering steps.

b

Excluded from analysis due to low yield of unique VH sequences.