Table 1.

Physiological characterization related parameters of the PHB-producing strains TMB4443 and TMB4425 under aerobic and anaerobic conditions, respectively

Strain	C-source	Nitrogen supply	Aeration	Initial CDW (g/L)	Yields						PHB titer (g/L)	PHB content (% PHB/CDW)
					YsX (g/g C-source)	YsXylOH (g/g C-source)	YsGlyOH (g/g C-source)	YsAcet (g/g C-source)	YsEtOH (g/g C-source)	YsPHB (mg/g C-source)
TMB4443	Glucose	No	Aerobic	3.8 ± 0.0	0.06 ± 0.00	0.00 ± 0.00	0.04 ± 0.01	0.01 ± 0.00	0.04 ± 0.01	1.0 ± 0.1	0.12 ± 0.00	1.8 ± 0.0
TMB4443	Glucose	Yes	Aerobic	0.01 ± 0.00	0.09 ± 0.01	0.00 ± 0.00	0.05 ± 0.01	0.03 ± 0.01	0.14 ± 0.07	2.12 ± 0.04	0.11 ± 0.00	2.4 ± 0.0
TMB4425	Xylose	No	Anaerobic	4.1 ± 0.2	0.06 ± 0.01	0.02 ± 0.00	0.09 ± 0.01	0.01 ± 0.00	0.01 ± 0.01	8.3 ± 0.8	0.43 ± 0.00	6.1 ± 0.1
TMB4425	Xylose	Yes	Anaerobic	0.02 ± 0.00	0.09 ± 0.00	0.04 ± 0.00	0.04 ± 0.00	0.01 ± 0.00	0.21 ± 0.03	13.8 ± 0.6	0.73 ± 0.00	16.4 ± 0.8

Cell dry weight (CDW), volumetric yields and titers were calculated from a single time-point corresponding to the end of the experiment (varied from 141 to 172 h). Values reported in the table represent the mean ± SD of at least two independent biological replicates. Glucose or xylose was used as sole C-source

Y_SX: biomass yield on C-source, Y_SXylOH: xylitol yield on C-source, Y_SGlyOH: glycerol yield on C-source, Y_SAcet: acetate yield on C-source, Y_SEtOH: ethanol yield on C-source and Y_SPHB: PHB yield on C-source. PHB titer: final PHB concentration. PHB content: percentage of PHB accumulated in the biomass formed