Figure 2.
Mice fed a western diet display increases in levels of 2-AG and anandamide in jejunum mucosa and plasma. Mice maintained on a standard diet (Stand) that were fasted for 24 h (FD) display increases in 2-AG levels in jejunum mucosa, when compared to free feeding controls [FF (a)], and FF male mice maintained on western diet (Western) display increases in levels of 2-AG in jejunum mucosa to levels found in fasted Stand mice (a). Western mice display elevated anandamide in jejunum mucosa when compared to Stand mice, irrespective of feeding condition (b). Western mice display increases in plasma levels of 2-AG (c) and anandamide (d) when compared to Stand mice. Two-way ANOVA with Student-Newman-Keuls multiple comparison post hoc test. * = p<0.05, *** = p<0.001, ns = not significant. Results are expressed as means ± SEM; n=7/condition for jejunum, n=9–10/condition for plasma.