Figure 3.

Inhibiting peripheral CB₁Rs reduces food intake in mice fed a western diet, but not a standard diet, and normalizes intake and meal patterns in western diet fed mice. Pharmacological blockade of peripheral CB₁Rs with AM6545, when compared to vehicle treatment (Veh), inhibits the caloric intake of male mice maintained for 60 days on western diet [WD (a)] during a 6 h test, but has no effect on caloric intake in mice maintained on standard chow diet [b (SD)]. Vehicle-treated WD mice display an increase in caloric intake (c), average meal size (d), and rate of feeding [e (kcal per minute of feeding)], when compared to Veh-treated SD mice during a 6 h test. Pharmacological blockade of peripheral CB₁Rs with AM6545 reduces total caloric intake (c), meal size (d), and rate of intake (e) in WD mice to levels indistinguishable from SD control mice, and has no effect on meal parameters in SD mice (c–e). Repeated measures (a, b) or regular (c–e) two-way ANOVA, with Sidak’s or Student-Newman-Keuls, respectively, multiple comparison post hoc test, *= p<0.05, ** = p<0.01, *** = p<0.001. Results are expressed as means ± SEM; n=7–8/condition.