Figure 4.
Expression of genes for components of the endocannabinoid system is modified in mice fed a western diet. Expression of mRNA encoding CB1R [a (CNR1)], CB2R [b (CNR2)], DAGL-α [c (DAGLA)], and FAAH (f) are elevated in standard chow-fed (Stand) 24 h fasted (FD) male mice, an effect absent in mice fed a western diet (Western). No changes are found under all conditions for DAGL-β [d (DAGLB)]. Expression of MAGL and FAAH genes are elevated in Western mice when compared to Stand (e, f). Two-Way ANOVA with Student-Newman-Keuls multiple comparison post hoc test. ** = p<0.01, * = p<0.05, ns = not significant. Results are expressed as means ± SEM; n=3–4/condition in triplicate.