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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: J Cell Biochem. 2016 Dec 29;118(4):748–753. doi: 10.1002/jcb.25728

Figure 1. Deletion of Raptor in prechondrogeic limb mesenchyme caused decreased size of limb bud cells.

Figure 1

Limb bud cells were isolated from E11.5 RapCKO embryos and their littermate controls, and then labeled with propidium iodide before FACS analysis. The Relative size of limb bud cells was determined using the parameter mean FSC-H. (A) Overlays of representative FSC-H histograms of RapCKO and control limb bud cells. (B) Mean FSC-H values (± S.D.) of RapCKO and control cells. n=3 for RapCKO embryos; n=4 for control embryos. **: P<0.001.