Figure 3. Disruption of mTORC1 signaling decreased chondrogenesis in primary limb bud micromass cultures.
(A–B) Alcian blue staining of wild-type limb bud micromass cultures after 4 days of culture in the absence (Veh, A) or the presence of 20 nM Rapamycin (Rapa, B). (C–D) Alcian blue staining of limb bud micromass cultures from E11.5 wild-type (C) and RapCKO (D) littermates. Scale bar: 2 mm.