FIGURE 4.

GmZOUs expressed in Arabidopsis zou-4 mutant. (A–D) Seed phenotype of GmZOU-1 and GmZOU-2 transgenic lines. The seeds from Col-0 are full, with the embryo showing a perfectly bent shape (A) and from zou-4 are abnormal with a shriveled seed phenotype (B). In GmZOU-1 transgenic lines, the seeds showed a wild-type-like or malformed seed phenotype (C), whereas the seeds in the GmZOU-2 transgenic lines showed the shriveled seed phenotype, similar to that in the Atzou-4 mutant (D). Scale bars: 1 mm. (E) qPCR analysis of the GmZOUs expression in transgenic lines. Four independent lines from each were selected for examining the exogenous GmZOUs expression, which was quantified relative to AtZOU expression in Col-0 wild type seeds at the heart stage. The un-transformed Atzou-4 mutants were selected as a negative control.