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. 2017 Feb 8;8:126. doi: 10.3389/fpls.2017.00126

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Copyright © 2017 Merelo, Agustí, Arbona, Costa, Estornell, Gómez-Cadenas, Coimbra, Gómez, Pérez-Amador, Domingo, Talón and Tadeo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunolocalization of pectic polysaccharides in the AZ-C. Longitudinal sections of tissue containing the AZ-C of Ricalate Navel maturing fruits were incubated with the monoclonal antibodies (mAb) LM5 (A,E,H,K), LM6 (B,F,I,L), and JIM5 (C,G,J,M) to detect (1,4)-β-D-galactans, (1,5)-α-L-arabinans and partially methylesterified/de-esterified HGs, respectively, after 0 (A, B and C), 24 (E,F,G) and 48 h (H–M) of ACC treatment. Control did not show immunofluorescence (D). Scale bars: 5 μm. Key labeling: walls at the two AZ-C cell areas (divided cells area [DCA] and starch-rich area [SA]) and at the fruit rind (FR) cell layers just below the SA showing fluorescence due to each of the mAbs after 0 (), 24 () or 48 () h of ACC treatment. Micrographs represent the merger of images from pectic epitopes detection by mAbs (green) and from cellulose detection by calcofluor white (blue).

Inline graphic — Immunolocalization of pectic polysaccharides in the AZ-C. Longitudinal sections of tissue containing the AZ-C of Ricalate Navel maturing fruits were incubated with the monoclonal antibodies (mAb) LM5 (A,E,H,K), LM6 (B,F,I,L), and JIM5 (C,G,J,M) to detect (1,4)-β-D-galactans, (1,5)-α-L-arabinans and partially methylesterified/de-esterified HGs, respectively, after 0 (A, B and C), 24 (E,F,G) and 48 h (H–M) of ACC treatment. Control did not show immunofluorescence (D). Scale bars: 5 μm. Key labeling: walls at the two AZ-C cell areas (divided cells area [DCA] and starch-rich area [SA]) and at the fruit rind (FR) cell layers just below the SA showing fluorescence due to each of the mAbs after 0 (), 24 () or 48 () h of ACC treatment. Micrographs represent the merger of images from pectic epitopes detection by mAbs (green) and from cellulose detection by calcofluor white (blue).