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. Author manuscript; available in PMC: 2018 Feb 15.
Published in final edited form as: J Immunol. 2017 Jan 6;198(4):1521–1530. doi: 10.4049/jimmunol.1601073

Figure 6. The inhibitory effect of PGE2 on IL-27 and irf1 expression is not mediated through STAT1/STAT2.

Figure 6

BMDC from WT 129S6 mice and Stat1−/− mice (A) or WT C57BL/6 mice and Stat2−/− mice (C) were stimulated with LPS or LPS+IFNγ in the presence or absence of PGE2 for 24hr. Supernatants were collected and subjected to IL-27 ELISA. ELISA data are representative of two independent experiments for Stat1−/− data and three independent experiments for Stat2−/− data. BMDC from WT 129S6 mice and Stat1−/− mice (B) or WT C57BL/6 mice and Stat2−/− mice (D) were stimulated with LPS or LPS+IFNγ in the presence or absence of PGE2 for 1h. Irf1 expression was determined by qRT-PCR. One of two independent experiments is shown. Results represent means ± SD. Statistics were calculated using one way ANOVA followed by Bonferroni correction (A, C) or unpaired t-test (B, D); *P<0.05, **P<0.01 and ***P<0.001.