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. 2017 Feb 8;8:96. doi: 10.3389/fimmu.2017.00096

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Copyright © 2017 Chandran, Heidu, Zelba, Schmid-Horch, Rammensee, Pascolo and Gouttefangeas.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Application of the bead assay to test in-house produced major histocompatibility complex (MHC) monomers. Two batches of conventional HLA-A*02 monomers (Batch 1 and Batch 2), each generated with the Melan A peptide (ELAGIGLTV) were tested. (A) PBMCs from two HLA-A02⁽⁺⁾ donors (indicated) were stained with APC-labeled MHC multimers generated from Batch 1 and PE-labeled MHC multimers generated from Batch 2. Multimer-APC/PE vs. CD8 PE-Cy7 dot plots are shown. (B) Fluorescence histograms obtained in the bead test.