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. 2017 Feb 8;7:29. doi: 10.3389/fcimb.2017.00029

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Copyright © 2017 Wang, Zhong, Wang, Song, Yu, Xing, Zhang, Yu, Hua and Yu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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EV-D68 infection induces G0/G1 arrest. (A,C,D) RD cells were infected with EV-D68 Fermon (A), US/MO/14-18947 (C) or US/KY/14-18953 (D) at an MOI of 0.8 or mock for 24 h for analysis of cell cycle status. Left: Cell-cycle profiles were determined by flow cytometry. Middle: The histograms show the percentage of cells in each phase of the cell cycle in mock-infected and EV-D68-infected cells. Right: The ratio of G0/G1 to G2/M is shown. (B) The virulence of Fermon, US/KY/14-18953 and US/MO/14-18947strains in infected RD cells was assessed 24 h post-infection with virus at MOI 0.8. Values are expressed as the TCID50/mL of the progeny viruses in the supernatant. The results represent the mean ± S.D of three independent experiments. ^**P < 0.01 and ^***P < 0.001.