FIG 4 .
Semiquantitative in situ analysis of rRNA and protein production along the dendrites. (a) GFP levels expressed from the dual rrnB promoters (strain SSB2020) were visualized by fluorescence microscopy at low resolution (×15) throughout an entire 1.5-cm monolayered dendrite. Selected images covering different regions of the community are shown. Semiquantitative analysis of fluorescence intensity per cell was carried out every 2.5 mm as indicated. Purple represents minimal expression, while the red-to-yellow gradient reflects the highest levels of fluorescence intensity. (b) GFP levels controlled by the transcriptional signals of the rpmGB gene encoding ribosomal protein L33 (strain OMG981). Analysis was as described for panel a.