FIG 5 .

The swarmers leading the community are actively making cell wall. Cells selectively removed from the base, the body, and the tip of an advancing dendrite (strain SSB2026) were pulse labeled with 7-hydroxycoumarin-amino-d-alanine (HADA). Incorporation of HADA is indicative of active peptidoglycan synthesis. (a) Fluorescence images show that HADA preferentially labels cells at the tip, integrating into the division septum, daughter cell poles, and the elongating peripheral wall. (b) Phase-contrast control images.