FIGURE 2.

(A–D) Effects of CORM-2 (A,B) or CORM-401 (C,D) on H₂O₂-induced intracellular total ROS production and cell death in MODE-K cells. MODE-K cells were treated for 40 min with H₂O₂ (1 mM) in the absence or presence of CORM-2 (40 μM) or CORM-401 (50 μM), added 1 h before H₂O₂. Intracellular total ROS levels were assessed with carboxy-H₂DCFDA (DCF), and gated on the viable, Sytox Red-negative population. Intracellular total ROS levels are expressed as % of control DCF MFI; cell death is expressed as % Sytox Red positivity. Control cells were incubated with serum-free medium alone; the effect of CORM-2 or CORM-401 per se was also tested. Mean ± SEM of three independent experiments. ^∗P < 0.05 vs. control. ^#P < 0.05 vs. H₂O₂ alone. (E,F) Influence of H₂O₂ on intracellular total ROS production and cell death, measured as described for (A–D). MODE-K cells were treated for 1 h with 2.5–10 mM H₂O₂. Control cells were incubated with serum-free medium alone. Mean ± SEM of three independent experiments. ^∗P < 0.05 vs. control.