FIGURE 5.

Effects of CORM-2 or CORM-401 on mitochondrial complex I (rotenone; Rot; A–D)- and mitochondrial complex III (antimycin-A; Ant-A; E–H)-induced mitochondrial ${\mathrm{O}}_{\mathrm{2}}^{\mathrm{•–}}$ production and cell death in MODE-K cells. MODE-K cells were treated for 6 h with rotenone (7.5 μM) or antimycin-A (10 μM) in the absence or presence of CORM-2 (40 μM; A,B,E,F) or CORM-401 (50 μM; C,D,G,H), added 1 h before rotenone or antimycin-A. Mitochondrial ${\mathrm{O}}_{\mathrm{2}}^{\mathrm{•–}}$ levels were assessed with MitoSOX Red, and gated on the viable, Sytox Red-negative population. Mitochondrial ${\mathrm{O}}_{\mathrm{2}}^{\mathrm{•–}}$ levels are expressed as % of control MitoSOX Red MFI; cell death is expressed as % Sytox Red positivity. Control cells were incubated with serum-free medium alone; the effect of CORM-2 or CORM-401 per se was also tested. Mean ± SEM of three independent experiments. ^∗P < 0.05 vs. control. ^#P < 0.05 vs. rotenone or antimycin-A alone.