Figure 2. Immunization with Tp0751 decreases severity of and bacterial load in primary lesions.

(a) Lesion ulceration and (b) lesion diameter were monitored over 14 days in control (N=2) and immunized (N=3) animals following T. pallidum challenge. (c) Bacterial load in lesions was assessed at day 10 PC by examining the presence of T. pallidum using darkfield microscopy. Results are presented as median±95% confidence interval between individual immunized (N=3) or control (N=2) animals. Significance was assessed using nonlinear regression and extra sum of squares F-test (**P<0.005 (a,b)) or a two-tailed t-test (*P<0.05 (c)).