Figure 5. DC MST1 signalling instructs Th17 differentiation in vitro.

(a) Naive CD4⁺T cells sorted from OT-II mice stimulated with antigen and LPS-pulsed CD8⁺DCs or CD11b⁺DCs from WT or Mst1^ΔDC mice for 5 days. Intercellular staining of IL-17 and IFNγ in T cells. Right, proportion of IL-17 and IFNγ in T cells. (b) Naive CD4⁺T cells sorted from C57BL/6 mice stimulated with anti-CD3 (2 μg ml⁻¹) and LPS-pulsed CD8⁺DCs or CD11b⁺DCs from WT or Mst1^ΔDC mice for 5 days. Intercellular staining of IL-17 and IFNγ in T cells was determined with FCM and a typical figure is shown. Right, proportion of IL-17 and IFNγ in T cells. (c) Naive T cells from C57BL/6 mice were co-cultured with WT or Mst1^ΔDC splenic DCs for 5 days in the presence of anti-CD3 and LPS. mRNA expression of the indicated genes in T cells were determined with qPCR. Levels in the WT control groups were set to 1. Data are representative of three to four independent experiments (mean±s.d.; n=4–6). **P<0.01 and ***P<0.001, compared with the indicated groups. P-values were determined using Student's t-tests.