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. 2017 Feb 1;8:14275. doi: 10.1038/ncomms14275

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(a) Intracellular staining of IL-6 in WT or Mst1^ΔDC splenic DCs 5 h after LPS stimulation in vitro. (Right) Proportion of IL-6 among the CD11c⁺ cells. The supernatant was collected and cytokine secretions determined with ELISA (b). (c,d) CD4⁺T cells from the indicated mice stimulated with WT or Mst1^ΔDC splenic DCs for 5 days. Intercellular staining of IL-17 and IFNγ in T cells was determined with FCM and a typical figure is shown. Right, proportion of IL-17 and IFNγ in T cells. Data are representative of three to four independent experiments (mean±s.d.; n=4–5). ***P<0.001, compared with the indicated groups. n.s., not significant. P-values were determined using Student's t-tests.