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. 2017 Feb 1;8:14275. doi: 10.1038/ncomms14275

Figure 7. T-cell IL-6R-p-STAT3 is responsible for DC MST1 signalling.

Figure 7

(a) Expression of IL-6Rα and IL-6Rβ (gp130) in T cells co-cultured with WT or Mst1ΔDC splenic DCs for 3 days. Right, the mean fluorescent intensity (MFI) is summarized. (b) Intercellular staining of p-STAT3 and p-STAT4 in T cells co-cultured with WT or Mst1ΔDC splenic DCs for 3 days. Right, MFI of the indicated protein is summarized. (c,d) Sorted CD4+ T cells were transfected with control or IL-6Rα (c) and IL-6Rβ (d) siRNA vector and stimulated with WT or Mst1ΔDC splenic DCs for 5 days. The intercellular staining of IL-17 and IFNγ in T cells performed with FCM and a typical figure shown. Right, the proportion of IL-17 and IFNγ in T cells. Data are representative of three to four independent experiments (mean±s.d.; n=4). *P<0.05, **P<0.01 and ***P<0.001, compared with the indicated groups. n.s., not significant. P-values were determined using Student's t-tests.