Figure 8. MST1 regulates IL-6 production through p38MAPK.
(a) Intercellular staining of the indicated proteins in splenic DCs following LPS stimulation in vitro. Expression of the phosphorylation of p38MAPK (MFI) among CD11c+cells is summarized in b. (c,d) Intracellular staining of IL-6 in DC isolated from the indicated mice 4 h after the i.p. injection of 10 mg kg−1 LPS (c). Right, the proportion of IL-6+ cells in DCs is summarized. The serum IL-6 level in the indicated mice (d). (e) Intracellular staining of IL-17 and IFNγ in T cells co-cultured with splenic DCs isolated from indicated mice was determined with FCM. Right, proportion of IL-17 and IFNγ in CD4+T cells is summarized. (f) Expression of the indicated gene in T cells was determined with FCM and the MFI summarized. Data are representative of four independent experiments (mean±s.d.; n=3–5). *P<0.05, **P<0.01 and ***P<0.001, compared with the indicated groups. P-values were determined using Student's t-tests.