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. 2017 Feb 1;8:14295. doi: 10.1038/ncomms14295

Figure 7. CAMKII and synapsin I phosphorylation and barr2/CAMKII co-immunoprecipitation/co-localization studies.

Figure 7

(a–f) Western blotting studies. (a–c) KCl (30 mM) stimulation of MIN6 cells treated with scrambled control siRNA (Con) promotes the phosphorylation of CAMKII (a,b) and synapsin I (a,c). These responses are abolished after treatment of MIN6 cells with barr2 siRNA. (b,c) Quantification of CAMKII and synapsin I phosphorylation data derived from immunoblotting experiments normalized by total CAMKII or total synapsin I expression, respectively. (d–f) Data represent means±s.e.m. of three independent experiments carried out in triplicate. *P<0.05, **P<0.01, as compared with the non-stimulated control group (one-way ANOVA followed by Tukey's post-test). (d–f) Western blotting experiments similar to those shown in a–c were carried out with pancreatic islets prepared from barr2-KO mice (KO) and control littermates (12-week-old males). (g) Total CAMKII expression levels in control and β-barr2 KO islets determined in immunoblotting studies using a pan-CAMKII antibody. Protein levels are expressed relative to the expression of β-actin. Data represent means±s.e.m. of three or four independent experiments (b,c,e–g). *P<0.05, as compared with the non-treated control group (one-way ANOVA followed by Tukey's post-test). ANOVA, analysis of variance.