Figure 1. The effects of in vitro griseofulvin treatment on P. falciparum growth.

(a) Inhibition of P. falciparum growth in griseofulvin-treated red blood cells, determined using the in vitro parasite growth assay. Red cells were incubated in the indicated concentrations of griseofulvin for three days prior to parasite inoculation and cultured for 54 h. Assays were conducted using P. falciparum strains 3D7 and K1. Data represent the mean (SD) of two independent assays (each concentration assayed in triplicate). Growth (b) and development (c) of P. falciparum 3D7 parasites in griseofulvin-treated red cells sampled at different times. Red blood cells were incubated in 50 μM griseofulvin for three days, and then inoculated with purified trophozoites. Total % parasitaemia and relative proportions of four parasite stages were determined just after inoculation (0 h) and at the indicated times. Assays were performed once for each time point.