Figure 2. Analysis of the griseofulvin cytotoxic effect on P. falciparum.

(a) N-MPP concentrations measured in P. falciparum-infected red blood cells (iRBC) and uninfected red blood cells (RBC) following treatment with griseofulvin treatment (100 μM), and in untreated iRBC. No N-MPP was detected in griseofulvin treated RBC or untreated iRBC. Data represent the mean (SD) of three independent assays. (b) Effect of different PPIX concentrations on the growth of P. falciparum incubated with griseofulvin (50 μM) or without griseofulvin (PPIX control). Assays were conducted for 54 h. Data represent mean (SEM) of two independent experiments (assayed in triplicate). *p < 0.001 by t-test and Bonferroni correction for multiple comparisons.