Figure 8. Nuclear Receptor Gene Expression Varies Between Pre-differentiated Cell Lines.

ATCC 3T3-L1, Zenbio 3T3-L1, and OP9 cells were cultured as described in Methods. Prior to induction of differentiation, RNA was extracted, cDNA prepared, and qPCR performed on a set of nuclear receptor genes involved in adipogenesis: peroxisome proliferator activated receptor α (PPARα, (A), PPARβ (B), PPARγ (C), liver X receptor α (LXRα; (D), LXRβ (E), glucocorticoid receptor (F), retinoic acid receptor α (RARα; (G), RARβ (H), RARγ (I), retinoid X receptor α (RXRα; (J), RXRβ (K), estrogen receptor α (ERα, (L), ERβ (M). Relative expression of each gene provided using 3T3-Swiss Albino cells from ATCC as the reference in three replicate assays. Different letters denote statistically different groups as per one-way ANOVA in GraphPad Prism.