Table 1. Comparison of Adipogenic Activity by Test Compounds.
| Chemical | Cell Line | EC20/IC20 (μM) | EC50/IC50 (μM) | Efficacy |
|---|---|---|---|---|
| Rosiglitazone | ATCC 3T3-L1 | 0.002 ± 3.1E-4 | 0.009 ± 2.2E-4 | 100.0% |
| Zenbio 3T3-L1 | 0.01 ± 0.9E-5 | 0.03 ± 1.1E-4 | 100.0% | |
| OP9 | 0.007 ± 1.1E-4 | 0.02 ± 1.2E-4 | 100.0% | |
| TBT | ATCC 3T3-L1 | 0.001 ± 1.5E-4 | 0.006 ± 5.6E-4 | 74.5% ± 9.6% |
| Zenbio 3T3-L1 | 0.001 ± 1.1E-4 | 0.003 ± 2.1E-4 | 15.6% ± 3.3% | |
| OP9 | 0.009 ± 1.5E-4 | 0.02 ± 3.8E-3 | 132.3% ± 17.6% | |
| T0070907 | ATCC 3T3-L1 | 1.67 ± 1.3E-4 | 3.06 ± 2.2E-4 | 91.9% ± 2.9% |
| Zenbio 3T3-L1 | 0.16 ± 1.2E-5 | 1.28 ± 4.8E-5 | 96.3% ± 0.3% | |
| OP9 | 0.62 ± 3.3E-5 | 1.53 ± 9.1E-5 | 100.0% ± 1.9% | |
| GW9662 | ATCC 3T3-L1 | 1.17 ± 0.13 | 2.52 ± 0.21 | 83.4% ± 10.0% |
| Zenbio 3T3-L1 | 1.25 ± 0.04 | 2.28 ± 0.09 | 81.9% ± 5.7% | |
| OP9 | 0.04 ± 3.9E-4 | 0.70 ± 1.1E-3 | 100.0% ± 0.8% | |
| BPA | ATCC 3T3-L1 | 0.58 ± 2.2E-4 | 2.15 ± 4.6E-3 | 23.0% ± 2.9% |
| Zenbio 3T3-L1 | N/A | N/A | 2.9% ± 0.3% | |
| OP9 | N/A | N/A | N/A | |
| TBBPA | ATCC 3T3-L1 | 0.27 ± 1.8E-4 | 1.68 ± 2.5E-4 | 45.7% ± 3.3% |
| Zenbio 3T3-L1 | 0.80 ± 1.2E-4 | 2.89 ± 2.2E-4 | 16.4% ± 3.7% | |
| OP9 | 1.33 ± 4.1E-5 | 3.44 ± 9.2E-4 | 28.4% ± 0.8% | |
| TCBPA | ATCC 3T3-L1 | 0.88 ± 2.4E-4 | 2.73 ± 0.27 | 46.3% ± 2.6% |
| Zenbio 3T3-L1 | 1.18 ± 0.09 | 3.23 ± 0.14 | 34.9% ± 15.4% | |
| OP9 | 0.85 ± 4.4E-5 | 2.83 ± 0.06 | 19.7% ± 0.6% | |
| BPAF | ATCC 3T3-L1 | 0.57 ± 0.06 | 2.10 ± 0.19 | 48.5% ± 10.7% |
| Zenbio 3T3-L1 | 1.25 ± 0.17 | 3.33 ± 0.08 | 33.3% ± 12.6% | |
| OP9 | N/A | N/A | N/A |
Values provided as the mean of three (or more) replicate assays ± standard error of the mean. Efficacy provided as the maximum relative percent agonist activity relative to the maximum rosiglitazone response or the maximum percent inhibition relative to the added agonist response for antagonists T0070907 and GW9662.
EC20 = concentration of test chemical (agonist) at which it exhibits 20% of its maximal activity.
EC50 = concentration of test chemical (agonist) at which it exhibits 50% of its maximal activity.
IC20 = concentration of test chemical (antagonist) at which it inhibits 20% of half-maximal rosiglitazone response.
IC50 = concentration of test chemical (antagonist) at which it inhibits 50% of half-maximal rosiglitazone response.
Rosiglitazone = PPARy positive control agonist, TBT = tributyltin chloride, T0070907 = adipogenesis antagonist control, GW9662 = PPARy antagonist control, BPA = bisphenol A, TBBPA = tetrabromobisphenol A, TCBPA = tetrachlorobisphenol A, BPAF = hexafluorobisphenol A.