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. 2017 Feb 8;7:42319. doi: 10.1038/srep42319

Figure 2. miR-664b-5p overexpression suppresses cell growth, migration and invasion.

Figure 2

(A) The efficiency of miR-664b-5p overexpression and inhibition in BRCA1-mutated TNBC cell lines was measured with qRT-PCR. (B) The influence of miR-664b-5p on the cell growth of BRCA1-mutated TNBC cells was measured with an MTT assay. (C) The representative images of cell apoptosis analysed by flow cytometry using Annexin V and PI staining. The apoptotic rate in MDA-MB-436 and HCC1937 cells transfected with miR-NC, miR-664b-5p, or anti-miR-664b-5p. (D) Representative images of the cell cycle analysis by flow cytometry using Annexin V and PI staining. The cell-cycle phase distribution of MDA-MB-436 and HCC1937 cells transfected with miR-NC, miR-664b-5p, or anti-miR-664b-5p. (E) Wound-healing assays in MDA-MB-436 and HCC1937 cells were performed after transduction with miR-NC, miR-664b-5p, or anti-miR-664b-5p. (F) Representative results of the Transwell assays showing the effect of miR-664b-5p expression on the invasion ability in MDA-MB-436 and HCC1937 cells. *p < 0.05.